Oocytes need to be kept at body temperature (36–37°C) as much as possible. There should also be appropriate equipment in use to maintain this temperature when laboratory and egg collection areas are on different sites. Scanning Petri dishes, collection tubes and heating blocks are pre-warmed at 37°C. Follicular aspirates are checked for the presence of oocyte-cumulus complexes under a stereo dissecting microscope with transmitted illumination base and heated stage. Exposure of oocytes to light should be minimized where possible.
Incubator temperature is one of the most precious variables in IVF labs. When the IVF started, almost all incubator temperatures were set at 37 degrees which mimic in vivo conditions. The majority of embryologists consider culture at 37 degrees is the back bone for the incubators. Embryology laboratories cover almost all IVFICSI-ET treatment needs with at least 2–3 incubators that should be checked daily for proper maintenance of temperature regulation. The absolute need for daily quality control of incubator temperature is justified by
1. The difficulty to maintain a stable temperature because of frequent opening of the incubator door
2. Overnight power failure where incubators indicate the set temperature and the external thermometer indicates a drop of more than 1 °C with deleterious effect on embryo cleavage and development and
3. Degrees of differences at different locations within the same incubator.
Prolonged exposure of cultures to temperatures other than optimal 37 °C Cheap Henrik Borgstrom Jersey
, reduces the ability of fertilization and nullifies the ability of cell division or cleavage, growth, implantation potential and subsequent achievement of pregnancy.
The temperature has to be maintained in dish used for embryos and other samples. This dish are specifically designed, made and tested for IVF laboratory so that the temperature inside the media of the dish be at 37 degree C when the stage is set at the same temperature. In all other dishes that are routinely used in the IVF lab Cheap Owen Tippett Jersey
, the temperature inside the dish will be well below 37 degree when the stage is set at 37 degree. This is due to the fact that there is an air gap of various distances, depending on the dish, between the bottom of the dish and the heated stage. Make it sure that heated stages maintain a temperature of 37 degree in the media inside the dish and not just 37 degree on the heated stage.
The control of the temperature of refrigerators and freezers where culture media is kept is extremely important. A lot of effort on monitoring the temperatures of heated stages and incubUnderfloor Water Heating Underfloor. Wholesale NFL Jerseys Wholesale Jerseys Wholesale Jerseys Wholesale Jerseys Cheap Jerseys Cheap Jerseys From China Cheap NFL Jerseys China Wholesale NFL Jerseys Free Shipping Wholesale NFL Jerseys From China Wholesale Basketball Jerseys